Huang Yu-Ling, Fan Ming-Xin, Wang Jin, Li Li, Lu Ning, Cao Yin-Xiang, Shen Lin-Lin, Zhu Da-Nian
Department of Physiology and Pathophysiology, Shanghai Medical College of Fudan University, Shanghai 200032, China.
Acupunct Electrother Res. 2005;30(3-4):263-73. doi: 10.3727/036012905815901235.
This study was to observe the changes of the neuronal and inducible nitric oxide synthase (nNOS & iNOS) as well as their mRNAs in the rostral ventrolateral medulla (RVLM) of stress-induced hypertensive rats before and after acupuncture, and thereby to infer the curative mechanism of acupuncture on hypertension. The result indicated that the systolic blood pressure (SBP) of stress group rats was increased significantly (P < 0.01), it was accompanied that the expression of nNOS in the RVLM, including the immunoreactive neuron number (P < 0.05), the optical density (OD) (P < 0.01), and the mRNA (P < 0.01) were obviously elevated, while those of iNOS (P < 0.05, P < 0.01 and P < 0.01) were evidently lowered in the stress-induced hypertensive rats. Electroacupuncture (EA) points at "Zusanli" (St. 36) and "Lanwei" (Extra 37) on the same hindlimb were stimulated by an EA apparatus (Type G6805-2) with dense sparse wave (4-20Hz) and 4mA intensity. EA application could return the SBP (P < 0.05), and the changes on the expression of both nNOS and iNOS (P < 0.05, P < 0.01 and P < 0.01). These results suggest that the curative mechanism of acupuncture on stress-induced hypertension is related to the changes of nNOS and iNOS in the RVLM of rats.
本研究旨在观察应激性高血压大鼠针刺前后延髓头端腹外侧区（RVLM）神经元型和诱导型一氧化氮合酶（nNOS和iNOS）及其mRNA的变化，从而推断针刺治疗高血压的作用机制。结果显示，应激组大鼠收缩压（SBP）显著升高（P<0.01），同时应激性高血压大鼠RVLM中nNOS的表达，包括免疫反应性神经元数量（P<0.05）、光密度（OD）（P<0.01）及mRNA（P<0.01）均明显升高，而iNOS的表达（P<0.05、P<0.01和P<0.01）则明显降低。采用G6805-2型电针仪，以疏密波（4~20Hz）、4mA强度刺激同一后肢的“足三里”（St.36）和“阑尾”（Extra 37）穴。电针可使SBP恢复正常（P<0.05），并使nNOS和iNOS的表达发生改变（P<0.05、P<0.01和P<0.01）。这些结果提示，针刺治疗应激性高血压的作用机制与大鼠RVLM中nNOS和iNOS的变化有关。
