[古尼虫草中真菌的分离与发酵培养]
[Isolation and fermentation culture of fungi from Cordyceps soofifera].
作者信息
Cheng Dong-qing, Ding Zhi-shan, Lin Mei-ai, Pan Pei-lei, Chen Yi-tao
机构信息
Zhejiang College of Traditional Chinese Medicine, Hangzhou 310053, China.
出版信息
Zhong Yao Cai. 2006 Feb;29(2):99-101.
OBJECTIVE
To study the fungi isolated from Cordyceps sobolifera and its fermentation culture.
METHODS
The fungi was isolated and identified by its hypha and spores. Three liquid media were used in the culture.
RESULTS
Pure culture was gained and the fungi was identified to be Paecilomyces cicadae. The fungi can grow best in liquid media: egg 110 g + silkworm powder 30 g + VB1 2 tahlets + MgSO4 x 7H2O 0.5 g + K2HPO4 1 g + H2O 1000 ml, in which every litre can produce 135 g wet hypha after cultured 4 d.
CONCLUSION
Paecilomyces cicadae from Cordyceps sobolifera can be cultured in liquid media.
目的
研究从蝉花及其发酵培养物中分离出的真菌。
方法
通过菌丝和孢子对真菌进行分离和鉴定。培养过程中使用了三种液体培养基。
结果
获得了纯培养物，鉴定该真菌为蝉拟青霉。该真菌在液体培养基中生长最佳：鸡蛋110克+蚕粉30克+维生素B1 2片+七水硫酸镁0.5克+磷酸氢二钾1克+水1000毫升，培养4天后，每升可产生135克湿菌丝。
结论
从蝉花中分离出的蝉拟青霉可在液体培养基中培养。
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