Zeng Zhaoying, Mou Dan, Luo Li, Zhong Wenlin, Duan Lin, Zou Xiao
Institute of Fungal Resources, College of Life Sciences, Guizhou University, Guiyang, China.
Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Guizhou Key Lab of Agro-Bioengineering, College of Life Sciences/Institute of Agro-Bioengineering, Guizhou University, Guiyang, China.
Front Microbiol. 2021 May 11;12:669785. doi: 10.3389/fmicb.2021.669785. eCollection 2021.
is an entomogenous fungus with important uses in traditional Chinese medicine. However, its wild resources have not met consumers' demand due to excessive harvesting practices. Artificial cultivation is therefore an important alternative, but research on cultivating in natural habitats has not been reported. In this study, we aimed to explore the viability of cultivating in a natural habitat, in the soil of forest. We assessed and compared the yield, metabolite contents and bacterial community composition of grown in the pupae at different growth stages, and under different cultivation conditions, in the soil of a natural habitat and in sterile glass bottles. Our results showed that cultivating in a natural habitat is feasible, with up to 95% of pupae producing fruiting bodies. The content of nitrogen compounds (amino acids) in cultivated in a natural habitat was significantly higher than in glass bottles, while the yield and carbon compound (mannitol and polysaccharide) and nucleoside (cordycepin and adenosine) contents were lower. Different bacterial genera were enriched in at different growth stages and cultivation environments, and these bacterial genera were closely related to metabolites contents during growth. This study demonstrated the viability of a novel cultivation method of , which could be used as an alternative to wild stocks of this fungus. These findings provided new insights into the growth mechanism of and its interaction with soil microorganisms.
是一种在传统中药中具有重要用途的虫生真菌。然而，由于过度采挖，其野生资源已无法满足消费者的需求。因此，人工栽培是一种重要的替代方法，但在自然栖息地进行栽培的研究尚未见报道。在本研究中，我们旨在探索在自然栖息地，即在[具体名称]森林土壤中栽培[具体名称]的可行性。我们评估并比较了在自然栖息地土壤和无菌玻璃瓶中，处于不同生长阶段、在不同栽培条件下培养于[具体名称]蛹体上的[具体名称]的产量、代谢物含量和细菌群落组成。我们的结果表明，在自然栖息地栽培[具体名称]是可行的，高达95%的蛹体产生了[具体名称]子实体。在自然栖息地栽培的[具体名称]中氮化合物（氨基酸）的含量显著高于玻璃瓶中的含量，而产量以及碳化合物（甘露醇和多糖）和核苷（虫草素和腺苷）的含量较低。在不同生长阶段和栽培环境下，不同的细菌属在[具体名称]中富集，并且这些细菌属与生长过程中的代谢物含量密切相关。本研究证明了一种新型[具体名称]栽培方法的可行性，该方法可作为这种真菌野生资源的替代品。这些发现为[具体名称]的生长机制及其与土壤微生物的相互作用提供了新的见解。
