Jones Jillian F, Whithear Kevin G, Scott Peter C, Noormohammadi Amir H
School of Veterinary Science, The University of Melbourne, Werribee, Victoria, 3052, Australia.
Avian Dis. 2006 Mar;50(1):88-91. doi: 10.1637/7429-083005R.1.
The minimum effective dose of the Mycoplasma synoviae-H (MS-H) vaccine was determined through protection against experimental challenge. Chickens were vaccinated by eyedrop with the following doses of a vaccine: 1.2 x 10(5), 2.4 x 10(5) 4.8 x 10(5), 9.6 x 10(5), 1.92 X 10(6), and 3.84 X 10(6) color change units (CCU), then challenged 6 wk after vaccination. Rapid serum agglutination results indicated that 100% of birds receiving an MS-H dose of > or = 4.8 x 10(5) CCU had antibodies to MS and enzyme-linked immunosorbent assay results showed that 60% of birds receiving a dose of 4.8 x 10(5) or 9.6 x 10(5) CCU and 100% of birds receiving a dose of 1.92 x 10(6) or 3.84 x 10(6) had antibodies to MS. At postmortem after challenge, the following parameters were significantly lower in birds vaccinated with an MS-H dose of > or = 4.8 x 10(5) CCU: air sac (AS) lesion severity; incidence of AS lesions; mucosal thicknesses in the upper trachea, middle trachea, and lower trachea (LT); and MS colonization of the LT and AS. It was concluded that an MS-H dose of 4.8 x 10(5) CCU was sufficient to elicit an antibody response in birds, prevent MS colonization in the LT and AS, and protect against AS lesions caused by an experimental MS and infectious bronchitis virus challenge.
通过对实验性攻毒的保护作用来确定滑膜支原体-H（MS-H）疫苗的最小有效剂量。给鸡滴眼接种以下剂量的疫苗：1.2×10⁵、2.4×10⁵、4.8×10⁵、9.6×10⁵、1.92×10⁶和3.84×10⁶个颜色变化单位（CCU），然后在接种后6周进行攻毒。快速血清凝集结果表明，接受MS-H剂量≥4.8×10⁵ CCU的鸡中100%具有抗MS抗体，酶联免疫吸附测定结果显示，接受4.8×10⁵或9.6×10⁵ CCU剂量的鸡中60%以及接受1.92×10⁶或3.84×10⁶剂量的鸡中100%具有抗MS抗体。攻毒后剖检时，接受MS-H剂量≥4.8×10⁵ CCU的鸡在以下参数方面显著较低：气囊（AS）病变严重程度；AS病变发生率；上呼吸道、中呼吸道和下呼吸道（LT）的黏膜厚度；以及LT和AS中的MS定植。得出的结论是，4.8×10⁵ CCU的MS-H剂量足以在鸡中引发抗体反应，防止MS在LT和AS中定植，并预防由实验性MS和传染性支气管炎病毒攻毒引起的AS病变。
