Björke Henrik, Andersson Karl
Division of Biomedical Radiation Sciences, Uppsala University, Sweden.
Appl Radiat Isot. 2006 Aug;64(8):901-5. doi: 10.1016/j.apradiso.2006.03.002. Epub 2006 Apr 18.
This report describes an automated method for the measurements of cellular retention and uptake of radiolabeled proteins interacting with cell-surface receptors on intact cancer cells. A complete uptake and retention measurement was performed in one cell dish using a rotating radioimmunoassay (RIA) principle. Compared to common manual measurements, rotating RIA saved both labor time and reagents and provided real-time binding traces with superior time-resolution. The rotating RIA retention profiles for different interactions agreed with retention times reported in the literature.
本报告描述了一种用于测量完整癌细胞上与细胞表面受体相互作用的放射性标记蛋白质的细胞保留和摄取的自动化方法。使用旋转放射免疫分析（RIA）原理在一个细胞培养皿中进行完整的摄取和保留测量。与常见的手动测量相比，旋转RIA节省了劳动时间和试剂，并提供了具有卓越时间分辨率的实时结合轨迹。不同相互作用的旋转RIA保留曲线与文献报道的保留时间一致。
