检测血浆中西尼罗河病毒RNA的核酸检测可提高有症状患者急性感染的快速诊断率。

Nucleic acid testing for west nile virus RNA in plasma enhances rapid diagnosis of acute infection in symptomatic patients.

作者信息

Tilley Peter A G, Fox Julie D, Jayaraman Gayatri C, Preiksaitis Jutta K

机构信息

Provincial Laboratory for Public Health (Microbiology), and Department of Microbiology and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada.

出版信息

J Infect Dis. 2006 May 15;193(10):1361-4. doi: 10.1086/503577. Epub 2006 Apr 4.

DOI:10.1086/503577

PMID:16619182

Abstract

Although nucleic acid amplification testing (NAAT) for West Nile virus (WNV) is useful in screening blood donors, such methods have not been studied in symptomatic patients. For diagnosis of WNV infection, 1.0 mL of plasma was tested by NAAT, and WNV-specific immunoglobulin M was assayed. Of 276 WNV cases, 191 were tested by both serology and NAAT. Of these, 86 (45.0%), 111 (58.1%), and 180 (94.2%) were detected by NAAT, serology, and combined NAAT and serology, respectively. NAAT-based screening was most useful within 8 days of the onset of symptoms. Viremia is common in early symptomatic WNV infection, and NAAT enhances diagnostic yield.

摘要

虽然西尼罗河病毒（WNV）的核酸扩增检测（NAAT）对筛查献血者很有用，但此类方法尚未在有症状的患者中进行研究。为诊断WNV感染，采用NAAT检测了1.0 mL血浆，并检测了WNV特异性免疫球蛋白M。在276例WNV病例中，191例同时进行了血清学检测和NAAT检测。其中，分别通过NAAT、血清学以及NAAT与血清学联合检测出86例（45.0%）、111例（58.1%）和180例（94.2%）。基于NAAT的筛查在症状出现后8天内最为有用。病毒血症在有症状的WNV感染早期很常见，NAAT可提高诊断率。