Andrade Coelho Claudia Alves, de Souza Nataly Araújo, Feder Maria Denise, da Silva Carlos Eugênio, Garcia Elói de Souza, Azambuja Patricia, Gonzalez Marcelo Salabert, Rangel Elizabeth F
Department of Entomology, Fundação Oswaldo Cruz, Av. Brasil, 4365, Manguinhos, Rio de Janeiro, RJ 21045-900, Brazil.
J Med Entomol. 2006 Mar;43(2):262-6. doi: 10.1603/0022-2585(2006)043[0262:eoaotd]2.0.co;2.
The effects of azadirachtin A added to the standard diet on the development, mortality, and metamorphosis of Lutzomyia longipalpis Lutz & Neiva, 1912 were studied. Concentrations of 0.1, 1.0, and 10.0 microg of azadirachtin/mg of diet significantly increased larval mortality in comparison with nontreated insects. Concentrations 0.1 and 1.0 microg blocked the molt of larvae, which remained as third instars until the end of the experiment. The 10 microg/mg concentration resulted in greater molt inhibition. In this group, all insects stopped their development as second instars. Simultaneous addition of ecdysone (1 microg/mg) to the standard diet containing azadirachtin counteracted the effects of azadirachtin on mortality and inhibition of ecdysis. These results indicate that azadirachtin is a potent growth inhibitor of L. longipalpis.
研究了在标准饲料中添加印楝素A对1912年路氏罗蛉发育、死亡率和变态的影响。与未处理的昆虫相比，饲料中印楝素浓度为0.1、1.0和10.0微克/毫克时显著增加了幼虫死亡率。0.1和1.0微克的浓度阻止了幼虫蜕皮，直到实验结束时它们仍处于三龄幼虫阶段。10微克/毫克的浓度导致更大程度的蜕皮抑制。在这一组中，所有昆虫都在二龄幼虫阶段停止发育。同时在含有印楝素的标准饲料中添加蜕皮激素（1微克/毫克）可抵消印楝素对死亡率和蜕皮抑制的影响。这些结果表明印楝素是路氏罗蛉的一种有效生长抑制剂。
