用于RRE IIB RNA-反式激活因子Rev肽相互作用检测的基于量子点的纳米传感器。
Quantum-dot-based nanosensor for RRE IIB RNA-Rev peptide interaction assay.
作者信息
Zhang Chun-yang, Johnson Lawrence W
机构信息
Department of Chemistry, York College and The Graduate Center, The City University of New York, Jamaica, New York 11451, USA.
出版信息
J Am Chem Soc. 2006 Apr 26;128(16):5324-5. doi: 10.1021/ja060537y.
Abstract
Rev is an important HIV-1 regulatory protein that binds the Rev responsive element (RRE) within the env gene of HIV-1 RNA genome; the binding of Rev to RRE is essential for the expression of the structural genes, gag-pol and env, and for HIV replication. Here we report a quantum-dot (QD)-based nanosensor that can be used in fluorescence resonance energy transfer (FRET) assays of RRE IIB RNA-Rev peptide interactions. In comparison with conventional fluorescent dye-based methods, this QD-based nanosensor offers the distinct advantages of not inhibiting the Rev-RRE interaction, high sensitivity, improved accuracy, and simultaneous FRET-related two-parameter detection. This QD-based nanosensor provides a new approach to study the effects of inhibitors upon Rev-RRE interaction, and it may have a wide applicability in the development of new drugs against HIV-1 infection.
摘要
Rev是一种重要的HIV-1调节蛋白，它能与HIV-1 RNA基因组env基因内的Rev反应元件（RRE）结合；Rev与RRE的结合对于结构基因gag-pol和env的表达以及HIV复制至关重要。在此，我们报告一种基于量子点（QD）的纳米传感器，可用于RRE IIB RNA与Rev肽相互作用的荧光共振能量转移（FRET）分析。与传统的基于荧光染料的方法相比，这种基于QD的纳米传感器具有不抑制Rev-RRE相互作用、高灵敏度、更高的准确性以及与FRET相关的双参数同时检测等显著优势。这种基于QD的纳米传感器为研究抑制剂对Rev-RRE相互作用的影响提供了一种新方法，并且在开发抗HIV-1感染的新药方面可能具有广泛的适用性。
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