Institute of Biomedical Engineering and Health Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.
Anal Chem. 2010 Mar 1;82(5):1921-7. doi: 10.1021/ac9026675.
Owing to their unique optical properties, quantum dots (QDs) with different colors have been applied for simultaneous detection of multiple analytes. However, the use of single QD for multiplex detection of analytes with single-molecule detection has not been explored. Here we report a single QD-based nanosensor for multiplex detection of HIV-1 and HIV-2 at single-molecule level in a homogeneous format. In this single QD-based nanosensor, the QD functions not only as a fluorescence pair for coincidence detection and as a fluorescence-resonance-energy-transfer (FRET) donor for FRET detection but also as a local nanoconcentrator which significantly amplifies the coincidence-related fluorescence signals and the FRET signals. This single-QD-based nanosensor takes advantage of a simple 'mix and detection' assay with extremely low sample consumption, high sensitivity, and short analysis time and has the potential to be applied for rapid point-of-care testing, gene expression studies, high-throughput screening, and clinical diagnostics.
由于其独特的光学特性，不同颜色的量子点 (QD) 已被应用于同时检测多种分析物。然而，尚未探索使用单个 QD 进行具有单分子检测的分析物的多重检测。在这里，我们报告了一种基于单个 QD 的纳米传感器，用于在均相格式中以单分子水平同时检测 HIV-1 和 HIV-2。在这个基于单个 QD 的纳米传感器中，QD 不仅用作符合检测的荧光对，而且用作荧光共振能量转移 (FRET) 供体用于 FRET 检测，还用作局部纳米浓缩器，可显著放大符合相关的荧光信号和 FRET 信号。这种基于单个 QD 的纳米传感器利用了一种简单的“混合和检测”测定法，具有极低的样品消耗、高灵敏度、短分析时间的优点，具有应用于快速即时护理检测、基因表达研究、高通量筛选和临床诊断的潜力。
