抗生素和抗菌肽对植物病原菌桑氏假单胞菌的体外活性。
In vitro activities of antibiotics and antimicrobial peptides against the plant pathogenic bacterium Xylella fastidiosa.
作者信息
Kuzina L V, Miller T A, Cooksey D A
机构信息
Department of Plant Pathology, University of California Riverside, Riverside, CA 92521, USA.
出版信息
Lett Appl Microbiol. 2006 May;42(5):514-20. doi: 10.1111/j.1472-765X.2006.01898.x.
AIMS
The objective of this study was to evaluate the effectiveness of antibiotics and antimicrobial peptides against 10 strains of Xylella fastidiosa.
METHODS AND RESULTS
The minimal inhibitory concentration (MIC) of 12 antibiotics and 18 antimicrobial peptides were determined by agar dilution tests and growth inhibition assays. Antibiotics with the lowest MIC for X. fastidiosa strains were gentamicin, tetracycline, ampicillin, kanamycin, and novobiocin, chloramphenicol, and rifampin. Plate growth inhibition assays showed that four of the antimicrobial peptides (Magainin 2, Indolicidin, PGQ, and Dermaseptin) were toxic to all X. fastidiosa strains.
CONCLUSION
All X. fastidiosa strains were sensitive to several groups of antibiotics, and minor differences in sensitivity to several antimicrobial peptides were observed among strains.
SIGNIFICANCE AND IMPACT OF THE STUDY
This study shows that antibiotics and antimicrobial peptides have some activity against the pathogen, X. fastidiosa and may have application in protecting plants from developing Pierce's disease.
目的
本研究的目的是评估抗生素和抗菌肽对10株桑萎蔫病菌的有效性。
方法与结果
通过琼脂稀释试验和生长抑制试验测定了12种抗生素和18种抗菌肽的最小抑菌浓度（MIC）。对桑萎蔫病菌株MIC最低的抗生素是庆大霉素、四环素、氨苄青霉素、卡那霉素、新生霉素、氯霉素和利福平。平板生长抑制试验表明，四种抗菌肽（蛙皮素2、吲哚杀菌素、PGQ和皮啡肽）对所有桑萎蔫病菌株都有毒性。
结论
所有桑萎蔫病菌株对几类抗生素敏感，且菌株间对抗菌肽的敏感性存在微小差异。
研究的意义与影响
本研究表明抗生素和抗菌肽对病原菌桑萎蔫病菌具有一定活性，可能在保护植物免受皮尔斯病侵害方面有应用价值。
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