由绿藻索氏小球藻产生的鞘的组成。
Composition of the sheath produced by the green alga Chlorella sorokiniana.
作者信息
Watanabe K, Imase M, Sasaki K, Ohmura N, Saiki H, Tanaka H
机构信息
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.
出版信息
Lett Appl Microbiol. 2006 May;42(5):538-43. doi: 10.1111/j.1472-765X.2006.01886.x.
AIMS
To investigate the chemical characterization of the mucilage sheath produced by Chlorella sorokiniana.
METHODS AND RESULTS
Algal mucilage sheath was hydrolysed with NaOH, containing EDTA. The purity of the hydrolysed sheath was determined by an ATP assay. The composition of polysaccharide in the sheath was investigated by high-performance anion-exchange chromatography with pulsed amperometric detection. Sucrose, galacturonic acid, xylitol, inositol, ribose, mannose, arabinose, galactose, rhamnose and fructose were detected in the sheath as sugar components. Magnesium was detected in the sheath as a divalent cation using inductively coupled argon plasma. The sheath matrix also contained protein.
CONCLUSIONS
It appears that the sheath is composed of sugars and metals. Mucilage sheath contains many kinds of saccharides that are produced as photosynthetic metabolites and divalent cations that are contained in the culture medium.
SIGNIFICANCE AND IMPACT OF THE STUDY
This is the first report on chemical characterization of the sheath matrix produced by C. sorokiniana.
目的
研究索氏小球藻产生的黏液鞘的化学特性。
方法与结果
用含乙二胺四乙酸（EDTA）的氢氧化钠水解藻类黏液鞘。通过ATP测定法测定水解鞘的纯度。采用高效阴离子交换色谱-脉冲安培检测法研究鞘中多糖的组成。在鞘中检测到蔗糖、半乳糖醛酸、木糖醇、肌醇、核糖、甘露糖、阿拉伯糖、半乳糖、鼠李糖和果糖作为糖成分。使用电感耦合氩等离子体在鞘中检测到镁作为二价阳离子。鞘基质中也含有蛋白质。
结论
黏液鞘似乎由糖和金属组成。黏液鞘包含多种作为光合代谢产物产生的糖类以及培养基中所含的二价阳离子。
研究的意义和影响
这是关于索氏小球藻产生的鞘基质化学特性的首次报道。
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