Hayashi T, Hayashi K, Maeda M, Kojima I
Faculty of Pharmaceutical Sciences and School of Medicine, Toyama Medical and Pharmaceutical University, Toyama, Japan.
J Nat Prod. 1996 Jan;59(1):83-7. doi: 10.1021/np960017o.
Bioactivity-directed fractionation of a hot H2O extract from a blue-green alga Spirulina platensis led to the isolation of a novel sulfated polysaccharide named calcium spirulan (Ca-SP) as an antiviral principle. This polysaccharide was composed of rhamnose, ribose, mannose, fructose, galactose, xylose, glucose, glucuronic acid, galacturonic acid, sulfate, and calcium. Ca-SP was found to inhibit the replication of several enveloped viruses, including Herpes simplex virus type 1, human cytomegalovirus, measles virus, mumps virus, influenza A virus, and HIV-1. It was revealed that Ca-SP selectively inhibited the penetration of virus into host cells. Retention of molecular conformation by chelation of calcium ion with sulfate groups was suggested to be indispensable to its antiviral effect.
对蓝藻钝顶螺旋藻热水提取物进行生物活性导向分级分离，得到一种名为钙螺旋藻多糖（Ca-SP）的新型硫酸化多糖作为抗病毒成分。这种多糖由鼠李糖、核糖、甘露糖、果糖、半乳糖、木糖、葡萄糖、葡萄糖醛酸、半乳糖醛酸、硫酸盐和钙组成。发现Ca-SP能抑制多种包膜病毒的复制，包括单纯疱疹病毒1型、人巨细胞病毒、麻疹病毒、腮腺炎病毒、甲型流感病毒和HIV-1。研究表明，Ca-SP能选择性抑制病毒进入宿主细胞。硫酸根与钙离子螯合保持分子构象被认为对其抗病毒作用必不可少。
