Cray C, Crouse C, Atherton S S, Levy R B
Department of Microbiology, University of Miami School of Medicine, Florida.
Arch Virol. 1991;121(1-4):101-10. doi: 10.1007/BF01316747.
Infection with cytomegalovirus is a major concern following bone marrow transplantation. Previously, we have described a model whereby infection by MCMV concurrent with the injection of parental strain lymphoid cells into F1 recipient mice results in a severe graft-versus-host reaction (GvHR) when the donor and host differ at the class I MHC locus (i.e., class I MHC disparate GvHR). The present studies were performed to determine whether one consequence of this severe GvHR is the alteration of the tissue distribution and/or levels of infectious virus as compared to mice not undergoing GvHR. Using PCR to detect MCMV DNA, it was observed that the tissue distribution of virus 3 days after injection was identical in recipients of virus alone and of GvHR plus virus inocula. At two weeks post injection, virus was recovered from the salivary gland and pancreas in both groups. In contrast to recipients of MCMV alone, lung tissue from GvHR plus virus mice also contained viral DNA. Notably, these results were corroborated by the plaque assay. Moreover, salivary gland and pancreas from recipients of GvHR plus virus were found to contain higher titers of infectious virus. In total, the data demonstrate that, with the notable exception of the lung, the distribution of virus was not changed in the animals despite the presence of a concurrent severe GvHR. However, viral replication in infected tissues appeared less restricted in these recipients.
巨细胞病毒感染是骨髓移植后的一个主要问题。此前，我们描述了一种模型，即当供体和宿主在I类主要组织相容性复合体（MHC）位点存在差异（即I类MHC不相合的移植物抗宿主反应，GvHR）时，将亲本株淋巴细胞注射到F1受体小鼠体内的同时感染小鼠巨细胞病毒（MCMV）会导致严重的移植物抗宿主反应。进行本研究以确定与未发生GvHR的小鼠相比，这种严重GvHR的一个后果是否是感染性病毒的组织分布和/或水平发生改变。使用聚合酶链反应（PCR）检测MCMV DNA，观察到单独接种病毒的受体和接种GvHR加病毒的受体在注射后3天病毒的组织分布相同。注射后两周，两组的唾液腺和胰腺中均回收了病毒。与单独接种MCMV的受体相比，接种GvHR加病毒的小鼠的肺组织也含有病毒DNA。值得注意的是，这些结果得到了噬斑测定的证实。此外，接种GvHR加病毒的受体的唾液腺和胰腺中发现含有更高滴度的感染性病毒。总体而言，数据表明，除了肺之外，尽管同时存在严重的GvHR，但动物体内病毒的分布并未改变。然而，在这些受体中，感染组织中的病毒复制似乎受到的限制较少。
