Electron paramagnetic resonance spectroscopy on isolated rat heart: a technical note.
作者信息
Gallo P, Colacicchi S, Ferrari M, Gualtieri G, Sotgiu A
机构信息
Department of Internal Medicine and Public Health, University of L'Aquila, Italy.
出版信息
Cardioscience. 1991 Dec;2(4):221-4.
PMID:1662085
Abstract
Spin labels have been measured directly in the isolated rat heart, perfused by the Langendorff method, using a 1500 MHz low frequency electron paramagnetic resonance spectrometer. This apparatus was previously employed to measure spin label pharmacokinetics of nitroxide sensitive to oxygen in whole mice. The heart was introduced in a resonant cavity to measure the uptake and clearance of free radicals. Various kinetic parameters were measured and are discussed, taking into account the features of the Langendorff apparatus.
摘要
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