Fei Xiao-Qing, Li Chen, Yu Xiao-Dong, Chen Hong-Yuan
Key Lab of Analytical Chemistry for Life Science, Ministry of Education, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, PR China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Jul 1;854(1-2):224-9. doi: 10.1016/j.jchromb.2007.04.031. Epub 2007 May 1.
This paper proposed a method with capillary zone electrophoresis (CZE) for analysis of eight heterocyclic amines in a commercial meat matrix. The influence of composition, pH and concentration of buffer, and applied voltage were investigated. A 5 mmol/L formic acid-ammonium formate solution at pH 2.20 was chosen as the running electrolyte. Also several solid-phase extraction actions were performed for the clean-up of the samples. With 3s hydrodynamic injection, detection limits ranging from 0.554 to 1.783 microg/g was obtained. To improve sensitivity, field-amplified sample injection (FASI) was used with the conditions of 3 s hydrodynamic injection of a water plug and 25 s electrokinetic injection of the sample. And methanol-water (1:1 in volume) was applied as the sample solvent. Under above conditions, detection limits ranged from 1.329 to 19.39 ng/g, which were at least 50 times lower than those with hydrodynamic injection.
本文提出了一种采用毛细管区带电泳（CZE）分析市售肉类基质中8种杂环胺的方法。研究了缓冲液的组成、pH值、浓度以及施加电压的影响。选择pH 2.20的5 mmol/L甲酸-甲酸铵溶液作为运行电解质。还进行了几次固相萃取操作以净化样品。采用3s的流体动力学进样，获得了0.554至1.783μg/g的检测限。为提高灵敏度，采用了场放大进样（FASI），条件为水塞3s流体动力学进样和样品25s电动进样。并将甲醇-水（体积比1:1）用作样品溶剂。在上述条件下，检测限为1.329至19.39 ng/g，比流体动力学进样时至少低50倍。
