McDowell John V, Hovis Kelley M, Zhang Hongming, Tran Emily, Lankford Justin, Marconi R T
Department of Microbiology and Immunology, Medical College of Virginia at Virginia Commonwealth University, Richmond, VA 23298-0678, USA.
Infect Immun. 2006 May;74(5):3030-4. doi: 10.1128/IAI.74.5.3030-3034.2006.
BBA68 (BbCRASP-1) of the Lyme disease spirochetes binds human factor H (FH) and FH-like protein 1 (FHL-1). Here we assess transcription of the BBA68 gene and production of BBA68 in infected mice and humans using real-time reverse transcriptase PCR and immunoblotting. The species specificity of FH binding to BBA68 was also tested. The data suggest that BBA68 does not play an important role in immune evasion in animals.
莱姆病螺旋体的BBA68（BbCRASP-1）可结合人补体因子H（FH）和类补体因子H蛋白1（FHL-1）。在此，我们运用实时逆转录聚合酶链反应和免疫印迹法评估了BBA68基因在受感染小鼠和人类中的转录情况以及BBA68的产生。同时还检测了FH与BBA68结合的物种特异性。数据表明，BBA68在动物的免疫逃逸中并不起重要作用。
