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在[32P - 腺苷酸]NAD存在的情况下对哺乳动物细胞蛋白质进行修饰：与幽门螺杆菌相关的ADP核糖基化活性的证据。

Modification of a mammalian cell protein in the presence of [32P-adenylate]NAD: evidence for ADP ribosylation activity associated with Helicobacter pylori.

作者信息

Nossa Carlos W, Blanke Steven R

机构信息

Department of Microbiology, University of Illinois, 601 South Goodwin Ave., Urbana, IL 61801, USA.

出版信息

Infect Immun. 2006 May;74(5):3071-6. doi: 10.1128/IAI.74.5.3071-3076.2006.

DOI:10.1128/IAI.74.5.3071-3076.2006
PMID:16622254
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1459732/
Abstract

Culture filtrates from Helicobacter pylori promote the transfer of the radiolabel from [32P-adenylate]NAD to one or more heat-labile factors within extracts prepared from several mammalian cell lines, with the predominate radiolabeled species exhibiting an apparent molecular mass of greater than 130 kDa. Our results suggest that several H. pylori strains release a factor that ADP-ribosylates a mammalian target protein.

摘要

幽门螺杆菌的培养滤液能促进放射性标记从[32P-腺苷酸]NAD转移至由几种哺乳动物细胞系制备的提取物中的一种或多种热不稳定因子，其中主要的放射性标记物种的表观分子量大于130 kDa。我们的结果表明，几种幽门螺杆菌菌株释放出一种能使哺乳动物靶蛋白发生ADP核糖基化的因子。

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