Bryant R J, Banks P M, O'Malley D P
Department of Pathology, Carolinas Medical Center, Charlotte, NC, USA.
Histopathology. 2006 Apr;48(5):505-15. doi: 10.1111/j.1365-2559.2006.02378.x.
To evaluate a group of lymphoid proliferations using only Ki67-stained slides to determine the value of the pattern of proliferating cells in diagnosis. Ki67 immunohistochemistry allows evaluation of the distribution of proliferating cells in addition to simply determining the proliferation rate of cells.
Three observers, using a Ki67-stained slide only, studied 149 cases from five diagnostic groupings: follicular hyperplasia, mixed pattern hyperplasia, localized Castleman's disease, follicular lymphoma and marginal zone lymphoma. The sensitivity for benign lesions varied from 94% to 97% among the three observers. Follicular lymphomas were recognized as neoplastic with a sensitivity of 96% and 100% by two of the observers. Marginal zone lymphoma was recognized as neoplastic in 67-73% of cases.
The Ki67 stain alone is a powerful tool for distinguishing benign from malignant proliferations within the selected groups. Nuances and pitfalls in the interpretation of Ki67 staining pattern are discussed.
仅使用Ki67染色切片评估一组淋巴样增生，以确定增殖细胞模式在诊断中的价值。Ki67免疫组化不仅能简单地确定细胞增殖率，还能评估增殖细胞的分布情况。
三名观察者仅使用Ki67染色切片，研究了来自五个诊断分组的149例病例：滤泡性增生、混合模式增生、局限性Castleman病、滤泡性淋巴瘤和边缘区淋巴瘤。三名观察者对良性病变的敏感性在94%至97%之间。两名观察者将滤泡性淋巴瘤识别为肿瘤性病变，敏感性分别为96%和100%。边缘区淋巴瘤在67%-73%的病例中被识别为肿瘤性病变。
仅Ki67染色是区分所选组内良性与恶性增殖的有力工具。文中讨论了Ki67染色模式解读中的细微差别和陷阱。
