Zuo Xue-lan, Zhou Xin, Meng Juan, Zhang Ke-jian, Liu Xiao-hong, Yang Hua-qiang
Department of Hematology, Zhongnan Hospital, Wuhan University, Wuhan 430071, China.
Zhonghua Nei Ke Za Zhi. 2006 Feb;45(2):133-5.
To explore the expression of myeloid cell leukemia 1 (MCL-1) proteins and survivin and its correlation with cell apoptosis as well as with the development and progression of B cell non-Hodgkin's lymphoma (B-NHL).
TdT-mediated dUTP nick end labeling and immunohistochemistry were used to study cell apoptosis and expression of MCL-1 proteins and survivin proteins in 43 patients with B-NHL and 10 with reactive hyperplasia (RH) lymphoid tissue.
The positive rate of MCL-1 proteins and survivin proteins was 58.1% (25/43) and 69.8% (30/43) respectively. The expression of MCL-1 proteins was not detected in RH lymphoid tissue, but that of survivin was detected in 10.0% (1/10). The expression of MCL-1 proteins in aggressive B-NHL was higher than that in indolent B-NHL (70.0 % vs 30.8 %, P < 0.05). The expression of survivin in aggressive B-NHL was also higher than that in indolent B-NHL (80.0% vs 46.2%, P < 0.05). Apoptotic index (AI) was not correlated positively with the expression of MCL-1, but correlated positively with the expression of survivin (r = 0.429, P < 0.01). MCL-1 and survivin were correlated positively in B-NHL (r = 0.598, P < 0.001).
MCL-1 proteins as family member of BCL-2 have no influence on apoptosis but survivin may participate in the regulation mechanism of B-NHL apoptosis. It is indicated that the two proteins with a close relationship may take part in the development and progression of B-NHL.
探讨髓样细胞白血病1（MCL-1）蛋白和生存素的表达及其与细胞凋亡以及B细胞非霍奇金淋巴瘤（B-NHL）发生、发展的关系。
采用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法及免疫组织化学方法，研究43例B-NHL患者及10例反应性增生（RH）淋巴组织中细胞凋亡情况以及MCL-1蛋白和生存素蛋白的表达。
MCL-1蛋白和生存素蛋白的阳性率分别为58.1%（25/43）和69.8%（30/43）。在RH淋巴组织中未检测到MCL-1蛋白的表达，但生存素的表达阳性率为10.0%（1/10）。侵袭性B-NHL中MCL-1蛋白的表达高于惰性B-NHL（70.0%对30.8%，P<0.05）。侵袭性B-NHL中生存素的表达也高于惰性B-NHL（80.0%对46.2%，P<0.05）。凋亡指数（AI）与MCL-1的表达无正相关，但与生存素的表达呈正相关（r=0.429，P<0.01）。在B-NHL中，MCL-1与生存素呈正相关（r=0.598，P<0.001）。
作为BCL-2家族成员的MCL-1蛋白对凋亡无影响，但生存素可能参与B-NHL凋亡的调控机制。提示这两种关系密切的蛋白可能参与B-NHL的发生、发展。
