Li Jiansha, Wu Huanming
Department of Pathology, School of Basic Medical Sciences, Tongli Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
J Huazhong Univ Sci Technolog Med Sci. 2006;26(5):504-7. doi: 10.1007/s11596-006-0504-9.
In order to investigate the expression change of survivin in non-Hodgkin lymphoma (NHL) and its possible effects on NHL development, the expression of survivin, Ki-67, caspase3 and FVIIIRAg in reactive lymphoid hyperplasia (RH) and NHL was detected by immunohistochemical assay, and apoptosis index (AI) in RH and NHL by TUNEL analysis. The results showed that the expression of survivin is significantly higher in aggressive NHL than in indolent NHL (P<0.01), while there was no statistically significant difference between RH and indolent NHL (P>0.05). The expression of survivin had a significantly positive correlation with the expression of Ki-67 and FVIIIRAg (r=0.6495, 0.6635, respectively, both P<0.01), and a negative correlation with the expression of caspase3 and AI (r=-0.5820, -0.6013, respectively, P<0.01). It was suggested that survivin may contribute to the progression of NHL by playing an important role in promoting cell proliferation, inhibiting cell apoptosis and enlisting angiogenesis. Survivin expression is closely related to malignant grade and therefore may be considered an important prognostic factor of NHL.
为探讨生存素（survivin）在非霍奇金淋巴瘤（NHL）中的表达变化及其对NHL发生发展的可能影响，采用免疫组化法检测反应性淋巴组织增生（RH）和NHL中survivin、Ki-67、半胱天冬酶3（caspase3）及FⅧ相关抗原（FVIIIRAg）的表达，并用TUNEL分析法检测RH和NHL中的凋亡指数（AI）。结果显示，侵袭性NHL中survivin的表达显著高于惰性NHL（P<0.01），而RH与惰性NHL之间无统计学差异（P>0.05）。survivin的表达与Ki-67和FVIIIRAg的表达呈显著正相关（r分别为0.6495、0.6635，均P<0.01），与caspase3的表达及AI呈负相关（r分别为-0.5820、-0.6013，P<0.01）。提示survivin可能通过促进细胞增殖、抑制细胞凋亡及参与血管生成等重要作用，推动NHL的进展。Survivin表达与恶性程度密切相关，因此可能是NHL的一个重要预后因素。
