Yuhi Teruko, Nagatani Naoki, Endo Tatsuro, Kerman Kagan, Takata Masayuki, Konaka Hiroyuki, Namiki Mikio, Takamura Yuzuru, Tamiya Eiichi
Japan Science and Technology Agency (JST) Innovation Plaza Ishikawa, 2-13 Asahidai, Nomi City, Ishikawa 923-1211, Japan.
J Immunol Methods. 2006 May 30;312(1-2):54-60. doi: 10.1016/j.jim.2006.02.011. Epub 2006 Mar 30.
A novel bioanalysis system based on immunochromatography was developed in connection with a nitrocellulose resin modified micropipette tip, such as ZipTip. The sandwich-type immunoassay was applied to our bioanalysis system. The simple handling of the micropipette enabled us to increase the sample volume and detect low concentrations of target antigens in urine samples. In addition, the washing procedure could also be performed easily to reduce the background signal levels. For analytical evaluations, the color intensity was captured by a flatbed scanner, and processed by a software. We have achieved the detection of human chorionic gonadotropin (hCG) and prostate-specific antigen (PSA). The detection limit of hCG was 0.5 ng/ml (0.05 ng/tip), which is comparable to that of other conventional immunochromatographic systems. Moreover, the detection of PSA was greatly improved over the existing systems with the application of different sample volumes, such as 1 ng/ml (0.2 ng/tip) in a 200 microl sample volume, and 1 ng/ml (0.3 ng/tip) in 300 microl sample volume. Our bioanalysis system is a promising candidate for application to point-of-care tests with its simple handling and high sensitivity.
开发了一种基于免疫层析的新型生物分析系统，该系统与硝酸纤维素树脂修饰的微量移液器吸头（如ZipTip）相关联。夹心型免疫测定法应用于我们的生物分析系统。微量移液器操作简单，使我们能够增加样品体积，并检测尿液样本中低浓度的目标抗原。此外，清洗过程也可轻松进行，以降低背景信号水平。为了进行分析评估，通过平板扫描仪捕获颜色强度，并由软件进行处理。我们已实现对人绒毛膜促性腺激素（hCG）和前列腺特异性抗原（PSA）的检测。hCG的检测限为0.5 ng/ml（0.05 ng/吸头），与其他传统免疫层析系统相当。此外，通过应用不同的样品体积，如200微升样品体积中的1 ng/ml（0.2 ng/吸头）和300微升样品体积中的1 ng/ml（0.3 ng/吸头），PSA的检测在现有系统基础上有了很大改进。我们的生物分析系统操作简单且灵敏度高，是即时检测应用的一个有前景的候选者。
