Seeley Stacy K, Poposki Julie A, Maksimchuk John, Tebbe Jill, Gaudreau Jon, Mannervik Bengt, Bull Arthur W
Department of Science and Math, Kettering University, Flint, MI 48504, USA.
Biochim Biophys Acta. 2006 Jul;1760(7):1064-70. doi: 10.1016/j.bbagen.2006.02.020. Epub 2006 Mar 31.
The oxidation of linoleic acid produces several products with biological activity including the hydroperoxy fatty acid 13-hydroperoxyoctadecadienoic acid (13-HPODE), the hydroxy fatty acid 13-hydroxyoctadecadienoic acid (13-HODE), and the 2,4-dienone 13-oxooctadecadienoic acid (13-OXO). In the present work, the peroxidase activity of glutathione transferases (GST) A1-1, M1-1, M2-2, and P1-1(Val 105) toward 13-HPODE has been examined. The alpha class enzyme is the most efficient peroxidase while the two enzymes from the mu class exhibit weak peroxidase activity toward 13-HPODE. It was also determined that the conjugated diene 13-HODE is not a substrate for GST from the alpha and mu classes but that 13-HODE does inhibit the GST-catalyzed conjugation of CDNB by enzymes from the alpha, mu, and pi classes. Finally, both 13-HODE and 13-OXO were shown to be inducers of GST activity in HT-29 and HCT-116 colon tumor cells. These data help to clarify the role of GST in the metabolic disposition of linoleic acid oxidation products.
亚油酸的氧化会产生几种具有生物活性的产物，包括氢过氧脂肪酸13-氢过氧十八碳二烯酸（13-HPODE）、羟基脂肪酸13-羟基十八碳二烯酸（13-HODE）以及2,4-二烯酮13-氧代十八碳二烯酸（13-OXO）。在本研究中，已检测了谷胱甘肽转移酶（GST）A1-1、M1-1、M2-2和P1-1（Val 105）对13-HPODE的过氧化物酶活性。α类酶是最有效的过氧化物酶，而μ类的两种酶对13-HPODE表现出较弱的过氧化物酶活性。还确定共轭二烯13-HODE不是α类和μ类GST的底物，但13-HODE确实会抑制α类、μ类和π类酶催化的CDNB共轭反应。最后，13-HODE和13-OXO均被证明是HT-29和HCT-116结肠肿瘤细胞中GST活性的诱导剂。这些数据有助于阐明GST在亚油酸氧化产物代谢处置中的作用。
