霍乱弧菌DNA促旋酶的纯化及性质

Mukhopadhyay G, Ghosh A

Indian Institute of Chemical Biology, Calcutta.

Biochem J. 1991 Dec 15;280 ( Pt 3)(Pt 3):797-800. doi: 10.1042/bj2800797.

The DNA gyrase was purified from Vibrio cholerae strain 569B. It appeared to be composed of two subunits of Mr 120,000 and 97,000 and had a great deal of similarity to the Escherichia coli gyrase. Unlike the E. coli enzyme, however, it could neither relax supercoiled DNA nor induce a cleavage of double-stranded DNA, under experimental conditions in which E. coli gyrase could do so.

DNA促旋酶是从霍乱弧菌569B菌株中纯化得到的。它似乎由分子量为120,000和97,000的两个亚基组成，并且与大肠杆菌促旋酶有很大的相似性。然而，与大肠杆菌的酶不同，在大肠杆菌促旋酶能够发挥作用的实验条件下，它既不能使超螺旋DNA松弛，也不能诱导双链DNA的切割。