通过荧光互补可视化分子相互作用。
Visualization of molecular interactions by fluorescence complementation.
作者信息
Kerppola Tom K
机构信息
Howard Hughes Medical Institute and Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan 48109-0650, USA.
出版信息
Nat Rev Mol Cell Biol. 2006 Jun;7(6):449-56. doi: 10.1038/nrm1929.
Abstract
The visualization of protein complexes in living cells enables the examination of protein interactions in their normal environment and the determination of their subcellular localization. The bimolecular fluorescence complementation assay has been used to visualize interactions among multiple proteins in many cell types and organisms. Modified forms of this assay have been used to visualize the competition between alternative interaction partners and the covalent modification of proteins by ubiquitin-family peptides.
摘要
活细胞中蛋白质复合物的可视化能够在其正常环境中检测蛋白质相互作用，并确定其亚细胞定位。双分子荧光互补分析已被用于在多种细胞类型和生物体中可视化多种蛋白质之间的相互作用。该分析的改进形式已被用于可视化替代相互作用伙伴之间的竞争以及泛素家族肽对蛋白质的共价修饰。
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