Ligios C, Cancedda M G, Madau L, Santucciu C, Maestrale C, Agrimi U, Ru G, Di Guardo G
Istituto Zooprofilattico Sperimentale della Sardegna, Sassari, Italy.
Arch Virol. 2006 Oct;151(10):2007-20. doi: 10.1007/s00705-006-0759-2. Epub 2006 Apr 20.
The pathogenesis of natural scrapie in Sarda breed sheep was investigated in 1050 asymptomatic and 49 sick sheep from scrapie-affected flocks. Central and peripheral nervous system, along with lymphoreticular system (LRS) tissues, were subjected to immunohistochemistry (IHC) and Western-blotting (WB) for detection of pathological isoform of the prion protein (PrP(Sc)). A total of 69 of the 1050 clinically healthy sheep were found to be infected with scrapie, with PrP(Sc) being detected in both the central nervous system (CNS) and enteric nervous system (ENS) plexuses of 60 of the sheep, while IHC and WB yielded evidence of (PrP(Sc)) deposition only in lymphoid tissues of the remaining 9 clinically healthy sheep. PrP(Sc) was also detected in the CNS, as well as in ENS plexuses from all of the 49 clinically affected sheep. Nevertheless, 18 of the 69 clinically healthy animals (26%, 17 ARQ/ARQ and 1 ARQ/AHQ sheep), along with 3 ARQ/ARQ sheep (6%) of the clinically affected group, showed no IHC or WB evidence of PrP(Sc) in lymphoid tissues, but PrP(Sc) could be still detected in their CNS and ENS plexuses. The study demonstrates dual CNS and ENS PrP(Sc) deposition in Sarda sheep with scrapie, in spite of an apparent lack of lymphoid tissue involvement in a number of cases.
在来自受羊瘙痒病影响的羊群的1050只无症状绵羊和49只患病绵羊中，对撒丁岛品种绵羊的自然羊瘙痒病发病机制进行了研究。对中枢和外周神经系统以及淋巴网状系统（LRS）组织进行免疫组织化学（IHC）和蛋白质印迹法（WB）检测，以检测朊病毒蛋白（PrP(Sc)）的病理异构体。在1050只临床健康的绵羊中，共有69只被发现感染了羊瘙痒病，其中60只绵羊的中枢神经系统（CNS）和肠神经系统（ENS）神经丛中均检测到PrP(Sc)，而在其余9只临床健康绵羊的淋巴组织中，免疫组织化学和蛋白质印迹法仅发现了PrP(Sc)沉积的证据。在所有49只临床患病绵羊的中枢神经系统以及肠神经系统神经丛中也检测到了PrP(Sc)。然而，69只临床健康动物中的18只（26%，17只ARQ/ARQ和1只ARQ/AHQ绵羊）以及临床患病组中的3只ARQ/ARQ绵羊（6%），在淋巴组织中未显示免疫组织化学或蛋白质印迹法检测到PrP(Sc)的证据，但在它们的中枢神经系统和肠神经系统神经丛中仍可检测到PrP(Sc)。该研究表明，尽管在一些病例中明显缺乏淋巴组织受累，但撒丁岛患有羊瘙痒病的绵羊存在中枢神经系统和肠神经系统双重PrP(Sc)沉积。
