Sontag W, Kalka D
Forschungszentrum Karlsruhe, Institut für Biologische Grenzflächen, Postfach 3640, 76021 Karlsruhe, Germany.
Radiat Environ Biophys. 2006 May;45(1):63-71. doi: 10.1007/s00411-006-0043-6. Epub 2006 Apr 20.
The effect of pulsed electromagnetic fields (PEMF) similar to those used in transcranial magnetic stimulation (TMS) on two tumour cell lines, the human promyelocytic leukaemia cell line (HL-60) and the rat pheochromocytoma cell line (PC12), was investigated. The two cell lines were exposed to non-homogeneous pulsed electromagnetic fields (about 0.25-4.5 T peak magnetic field strength; 1-8 exponential pulses, 0.25 Hz) at different positions on the coil (2x25 mm). After exposure with various intensities, various numbers of pulses and at different coil positions, cell viability and the intracellular cyclic AMP content were determined in the two cell lines. Additionally, in HL-60 cells the intracellular Hsp72 content and in PC12 cells the release of the neurotransmitters dopamine, noradrenaline and acetylcholine were measured after PEMF treatment. The results of these analyses do not hint at alterations in the cell viability or in the content of cAMP, Hsp72, dopamine, noradrenaline, and acetylcholine in the two tumour cell lines after PEMF exposure under various conditions.
研究了类似于经颅磁刺激（TMS）中使用的脉冲电磁场（PEMF）对两种肿瘤细胞系，即人早幼粒细胞白血病细胞系（HL-60）和大鼠嗜铬细胞瘤细胞系（PC12）的影响。将这两种细胞系置于线圈上不同位置（2×25毫米），暴露于非均匀脉冲电磁场（峰值磁场强度约为0.25 - 4.5特斯拉；1 - 8个指数脉冲，0.25赫兹）。在以不同强度、不同脉冲数并在不同线圈位置进行暴露后，测定了这两种细胞系的细胞活力和细胞内环磷酸腺苷（cAMP）含量。此外，在HL-60细胞中测量了细胞内热休克蛋白72（Hsp72）含量，在PC12细胞中测量了脉冲电磁场处理后神经递质多巴胺、去甲肾上腺素和乙酰胆碱的释放。这些分析结果并未表明在各种条件下脉冲电磁场暴露后，这两种肿瘤细胞系的细胞活力或cAMP、Hsp72、多巴胺、去甲肾上腺素和乙酰胆碱的含量有改变。
