Zeng Shaoqun, Lv Xiaohua, Zhan Chen, Chen Wei R, Xiong Wenhui, Jacques Steven L, Luo Qingming
National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China.
Opt Lett. 2006 Apr 15;31(8):1091-3. doi: 10.1364/ol.31.001091.
The dispersive nature of the acousto-optical deflector (AOD) presents a challenge to applications of two sequential orthogonal AODs (a two-dimensional AOD) as XY scanners in multiphoton microscopy. Introducing a prism before the two-dimensional (2D) AOD allows both temporal and spatial dispersion to be compensated for simultaneously. A 90 fs laser pulse was broadened to 572 fs without compensation, and 143 fs with compensation. The ratio of long axis to short axis of the exiting laser beam spot was 3.50 without compensation and 1.14 with compensation. The insertion loss was 37%. Two-photon fluorescence microscopy used the compensated 2D AOD scanner to image a fluorescent microsphere, which improves signal intensity -15-fold compared with the uncompensated scanner.
声光偏转器（AOD）的色散特性给将两个连续正交AOD（二维AOD）用作多光子显微镜中的XY扫描器的应用带来了挑战。在二维（2D）AOD之前引入一个棱镜可以同时补偿时间和空间色散。一个90飞秒的激光脉冲在未补偿时展宽到572飞秒，在补偿后展宽到143飞秒。出射激光束光斑的长轴与短轴之比在未补偿时为3.50，在补偿后为1.14。插入损耗为37%。双光子荧光显微镜使用补偿后的2D AOD扫描器对荧光微球进行成像，与未补偿的扫描器相比，信号强度提高了15倍。
