Reddy Gaddum Duemani, Saggau Peter
Rice University, Department of Bioengineering, Houston, Texas 77005, USA.
J Biomed Opt. 2005 Nov-Dec;10(6):064038. doi: 10.1117/1.2141504.
A scheme for fast 3-D laser scanning using acousto-optic deflectors is proposed and demonstrated. By employing counterpropagating acoustic waves that are both chirped and offset in their frequencies, we show that it is possible to simultaneously scan both axially and laterally with frame rates on the order of tens of kilohertz. This scheme was specifically designed for application with multiphoton imaging, particularly of neurons, where it will enable the concurrent monitoring of physiological signals at multiple locations within a microscopic 3-D volume (350 x 350 x 200 microm). When used for this purpose, we demonstrate how this scheme would also inherently compensate for spatial dispersion when ultrafast laser pulses are used in acousto-optic multiphoton microscopy.
提出并演示了一种使用声光偏转器进行快速三维激光扫描的方案。通过采用频率既啁啾又有偏移的反向传播声波，我们表明可以以数十千赫兹的帧率同时进行轴向和横向扫描。该方案专为多光子成像应用而设计，特别是神经元成像，它将能够在微观三维体积（350×350×200微米）内的多个位置同时监测生理信号。当用于此目的时，我们展示了该方案在声光多光子显微镜中使用超快激光脉冲时如何固有地补偿空间色散。
