Sieunarine Krishen, Zakaria Faris B P, Boyle Deborah C M, Corless David J, Noakes David E, Lindsay Iain, Lawson Andrew, Ungar Laszlo, Del Priores Giuseppe, Smith J Richard
Department of Gynaecology, Chelsea and Westminster Hospital at Imperial College School of Medicine, London, United Kingdom.
Int Surg. 2005 Nov-Dec;90(5):249-56.
Previously published work concluded that uterine artery microvascular anastomosis in the porcine model was feasible with subsequent normal vascular function in pregnancy. of the anastomosed vessels. The objective of this study was to assess the feasibility of uterine autotransplantation in a porcine model using microvascular anastomoses. Eight large white/landrace sows of proven fertility were used. A supracervical hysterectomy with or without bilateral salpingo-oophorectomy (BSO) was performed. After 1 hour of cold storage in a transplant solution, the specimen was reintroduced and followed by stepwise vascular reanastomosis. Objective perfusion index measurements suggested adequate uterine perfusion after transplantation. Postoperatively, sow 1 survived to 3 months with no signs of oestrus, and sows 2, 6, and 8 were killed on days 6, 33, and 54, respectively, for pelvic infection. Histopathology of the uterine grafts revealed gradual vessel thromboses. Microvascular reanastomosis is feasible, however, unsuccessful in uterine autotransplantation because of gradual vessel thromboses. The porcine model is highly susceptible to postoperative infection.
先前发表的研究得出结论，在猪模型中进行子宫动脉微血管吻合术是可行的，吻合后的血管在妊娠期具有正常的血管功能。本研究的目的是评估使用微血管吻合术在猪模型中进行子宫自体移植的可行性。选用了8头经证实具有生育能力的大白/长白母猪。进行了子宫颈上子宫切除术，可选择同时或不同时进行双侧输卵管卵巢切除术（BSO）。在移植溶液中冷藏1小时后，重新植入标本，随后进行逐步的血管再吻合。客观灌注指数测量表明移植后子宫灌注充足。术后，母猪1存活至3个月，无发情迹象，母猪2、6和8分别在第6天、33天和54天因盆腔感染而被处死。子宫移植物的组织病理学显示血管逐渐形成血栓。微血管再吻合术是可行的，然而，由于血管逐渐形成血栓，子宫自体移植未成功。猪模型对术后感染高度敏感。
