RNA聚合酶I转录机制。
The RNA polymerase I transcription machinery.
作者信息
Russell Jackie, Zomerdijk Joost C B M
机构信息
Division of Gene Regulation and Expression, School of Life Sciences, University of Dundee, Wellcome Trust Biocentre, Dundee DD1 5EH, UK.
出版信息
Biochem Soc Symp. 2006(73):203-16. doi: 10.1042/bss0730203.
Abstract
The rRNAs constitute the catalytic and structural components of the ribosome, the protein synthesis machinery of cells. The level of rRNA synthesis, mediated by Pol I (RNA polymerase I), therefore has a major impact on the life and destiny of a cell. In order to elucidate how cells achieve the stringent control of Pol I transcription, matching the supply of rRNA to demand under different cellular growth conditions, it is essential to understand the components and mechanics of the Pol I transcription machinery. In this review, we discuss: (i) the molecular composition and functions of the Pol I enzyme complex and the two main Pol I transcription factors, SL1 (selectivity factor 1) and UBF (upstream binding factor); (ii) the interplay between these factors during pre-initiation complex formation at the rDNA promoter in mammalian cells; and (iii) the cellular control of the Pol I transcription machinery.
摘要
核糖体RNA（rRNA）构成核糖体的催化和结构成分，而核糖体是细胞的蛋白质合成机器。因此，由RNA聚合酶I（Pol I）介导的rRNA合成水平对细胞的生命和命运具有重大影响。为了阐明细胞如何实现对Pol I转录的严格控制，使rRNA的供应在不同细胞生长条件下与需求相匹配，了解Pol I转录机器的组成部分和机制至关重要。在这篇综述中，我们将讨论：（i）Pol I酶复合物以及两个主要的Pol I转录因子，即选择性因子1（SL1）和上游结合因子（UBF）的分子组成和功能；（ii）在哺乳动物细胞的核糖体DNA（rDNA）启动子处形成起始前复合物的过程中，这些因子之间的相互作用；以及（iii）对Pol I转录机器的细胞调控。
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