γ-氨基丁酸A受体β3亚基的混杂性，表现为其存在于含α1、α2和α3亚基的受体亚群中。
Promiscuity of GABAA-receptor beta 3 subunits as demonstrated by their presence in alpha 1, alpha 2 and alpha 3 subunit-containing receptor subpopulations.
作者信息
Pollard S, Duggan M J, Stephenson F A
机构信息
Department of Pharmaceutical Chemistry, School of Pharmacy, London, UK.
出版信息
FEBS Lett. 1991 Dec 16;295(1-3):81-3. doi: 10.1016/0014-5793(91)81390-t.
Polyclonal antibodies were raised in rabbits against the GABAA-receptor beta 3 subunit peptide sequence, KQSMPREGHGRHMDR-NH2 coupled to keyhole limpet haemocyanin. These anti-beta 3 379-393 antibodies immunoprecipitated in a dose-dependent manner specific benzodiazepine agonist binding sites from Na+ deoxycholate extracts of bovine cerebral cortex. In immunoblots, anti-beta 3 379-393 antibodies recognised two species with Mr 59,900 and Mr 57,200 in all preparations tested, which included crude detergent-solubilised, benzodiazepine affinity chromatography-purified receptor, anti-alpha 1 324-341 antibody, anti-Cys alpha 2 414-424 antibody and anti-Cys alpha 3 454-467 antibody immunoaffinity-purified GABAA-receptor subpopulations. These results provide evidence for the ubiquity and promiscuity of the GABAA-receptor beta 3 subunit.
用与钥孔血蓝蛋白偶联的GABAA受体β3亚基肽序列KQSMPREGHGRHMDR-NH2在兔体内制备多克隆抗体。这些抗β3 379-393抗体以剂量依赖的方式从牛脑皮质的Na+脱氧胆酸盐提取物中免疫沉淀特异性苯二氮䓬激动剂结合位点。在免疫印迹中，抗β3 379-393抗体在所有测试制剂中识别出两种分子量分别为59,900和57,200的蛋白条带，这些制剂包括粗去污剂溶解的、苯二氮䓬亲和层析纯化的受体、抗α1 324-341抗体、抗半胱氨酸α2 414-424抗体和抗半胱氨酸α3 454-467抗体免疫亲和纯化的GABAA受体亚群。这些结果为GABAA受体β3亚基的普遍存在和混杂性提供了证据。
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