Rusca T A, Kennedy P G, Bruns T D
Department of Plant and Microbial Biology, 111 Koshland Hall, University of California, Berkeley, CA 94720-3102, USA.
New Phytol. 2006;170(3):551-60. doi: 10.1111/j.1469-8137.2006.01689.x.
Our primary goal was to determine whether detection of Rhizopogon internal transcribed spacer (ITS) groups is affected by the pine species used in seedling bioassays. Our secondary goal was to investigate composition of Rhizopogon spore banks in the Eastern Sierra Nevada of California, a previously unsampled region. We used seedlings of Pinus contorta, Pinus jeffreyi, Pinus lambertiana, and Pinus muricata as bioassay plants and identified the Rhizopogon retrieved by internal transcribed spacer (ITS) sequence analysis. We found that each of the pine species retrieved all of the abundant Rhizopogon ITS groups, but there were significant differences among pines in the richness of Rhizopogon ITS groups recovered. Pinus muricata recovered all ITS groups found in this study and was significantly better than P. lambertiana. Rhizopogon communities from the five sampled sites contained six to eight ITS groups per site, with two unique sequence groups and a higher abundance of the Rhizopogon ellenae and Rhizopogon arctostaphyli groups than at previously sampled sites. These results show high cross-receptivity between Rhizopogon and pine species, and regional patterns in spore bank composition.
我们的主要目标是确定幼苗生物测定中使用的松树种类是否会影响对须腹菌内转录间隔区（ITS）类群的检测。我们的次要目标是调查加利福尼亚州内华达山脉东部一个此前未采样地区的须腹菌孢子库组成。我们使用扭叶松、杰弗里松、糖松和滨海松的幼苗作为生物测定植物，并通过内转录间隔区（ITS）序列分析鉴定回收的须腹菌。我们发现，每种松树都回收了所有丰富的须腹菌ITS类群，但在回收的须腹菌ITS类群丰富度方面，松树之间存在显著差异。滨海松回收了本研究中发现的所有ITS类群，并且明显优于糖松。来自五个采样地点的须腹菌群落每个地点包含6至8个ITS类群，有两个独特的序列类群，并且须腹菌埃氏类群和须腹菌熊果类群的丰度高于此前采样地点。这些结果表明须腹菌与松树种类之间具有高度交叉接受性，以及孢子库组成的区域模式。
