Leonenko Z, Finot E, Cramb D
Department of Chemistry, University of Calgary, 2500 University Drive NW, Calgary, Canada AB T2N 1N4.
Biochim Biophys Acta. 2006 Apr;1758(4):487-92. doi: 10.1016/j.bbamem.2006.02.033. Epub 2006 Mar 31.
In spite of numerous investigations, the molecular mechanism of general anesthetics action is still not well understood. It has been shown that the anesthetic potency is related to the ability of an anesthetic to partition into the membrane. We have investigated changes in structure, dynamics and forces of interaction in supported dipalmitoylphosphatidylcholine (DPPC) bilayers in the presence of the general anesthetic halothane. In the present study, we measured the forces of interaction between the probe and the bilayer using an atomic force microscope. The changes in force curves as a function of anesthetic incorporation were analyzed. Force measurements were in good agreement with AFM imaging data, and provided valuable information on bilayer thickness, structural transitions, and halothane-induced changes in electrostatic and adhesive properties.
尽管进行了大量研究，但全身麻醉药作用的分子机制仍未得到充分理解。已经表明，麻醉效能与麻醉药分配到膜中的能力有关。我们研究了在全身麻醉药氟烷存在下，支持的二棕榈酰磷脂酰胆碱（DPPC）双层膜的结构、动力学和相互作用力的变化。在本研究中，我们使用原子力显微镜测量了探针与双层膜之间的相互作用力。分析了作为麻醉药掺入量函数的力曲线变化。力测量结果与原子力显微镜成像数据高度一致，并提供了有关双层膜厚度、结构转变以及氟烷引起的静电和粘附特性变化的有价值信息。
