Sanchez B, Robledo M M, Yelamos J, Gonzalez M F, Magariño R, Bermudo M P, Nuñez-Roldan A
Servicio de Immunología, Hospital Universitario Virgen del Rocío, Sevilla, Spain.
J Immunol Methods. 1991 Dec 15;145(1-2):193-7. doi: 10.1016/0022-1759(91)90326-b.
B cells from the peripheral blood of chronic lymphocytic leukemia patients were isolated by gradient density centrifugation and used without irradiation as a feeder layer in the cloning of human heterohybridoma cell lines by limiting dilution. Cloning efficiencies were high with all the cell lines tested. These feeder leukemia B cells could also be successfully used after having been stored in liquid nitrogen.
通过梯度密度离心法从慢性淋巴细胞白血病患者的外周血中分离出B细胞，在通过有限稀释法克隆人异源杂交瘤细胞系时，将其作为饲养层使用，且无需辐照。所有测试的细胞系克隆效率都很高。这些饲养性白血病B细胞在液氮中保存后也能成功使用。
