Brodin T, Olsson L, Sjögren H O
J Immunol Methods. 1983 May 27;60(1-2):1-7. doi: 10.1016/0022-1759(83)90329-0.
Human monocytes were prepared from peripheral blood by buoyant density centrifugation and subsequent absorption-elution on a column of gelatin beads. The eluted fraction containing 60-80% monocytes was used as feeder layer in cloning of the human lymphoma line RH-L4, the human myeloma line SKO-007, and a human hybridoma cell derived from the latter line. Cloning efficiencies were high in both liquid and semisolid media with all 3 cell lines tested. Feeder monocytes could also be successfully used after having been stored in liquid nitrogen.
通过浮力密度离心法从外周血中制备人单核细胞，随后在明胶珠柱上进行吸附 - 洗脱。将含有60 - 80%单核细胞的洗脱组分用作人淋巴瘤细胞系RH - L4、人骨髓瘤细胞系SKO - 007以及源自后一种细胞系的人杂交瘤细胞克隆中的饲养层。在所有测试的3种细胞系中，在液体和半固体培养基中的克隆效率都很高。饲养单核细胞在液氮中储存后也能成功使用。
