Jaimes Claudia P, Bernal M Mauricio, Suárez Zulma R, Montoya Dolly
Instituto de Biotecnología, Universidad Nacional de Colombia A.A. 14490, Colombia.
J Microbiol Methods. 2006 Oct;67(1):64-9. doi: 10.1016/j.mimet.2006.03.002. Epub 2006 Apr 19.
Amplified Fragment Length Polymorphism (AFLP) analysis was used for characterising 13 native Colombian Clostridium spp strains. The DNA extraction method was optimised and the use of cetyl trimethyl ammonium bromide (CTAB) and sodium chloride (NaCl) was incorporated. All strains could be typed in these conditions. The AFLP profiles obtained were submitted to multivariate analysis and compared with previous pulsed field gel electrophoresis (PFGE) results. The results suggested that the set of native strains could correspond to two new species different to those having been described to date. It is proposed that DNA-DNA hybridisation analysis should be done to produce complementary information for describing the new species.
扩增片段长度多态性（AFLP）分析用于鉴定13株哥伦比亚本土梭菌属菌株。对DNA提取方法进行了优化，并加入了十六烷基三甲基溴化铵（CTAB）和氯化钠（NaCl）。在这些条件下所有菌株都可以分型。将获得的AFLP图谱进行多变量分析，并与之前的脉冲场凝胶电泳（PFGE）结果进行比较。结果表明，这组本土菌株可能对应于两个与迄今已描述的物种不同的新物种。建议进行DNA-DNA杂交分析以提供补充信息来描述这些新物种。
