Keller J, Wieland B, Wittwer M, Stephan R, Perreten V
Institute of Veterinary Bacteriology, University of Bern, Länggassstrasse 122, CH-3001 Bern, Switzerland.
Zoonoses Public Health. 2007;54(1):2-7. doi: 10.1111/j.1863-2378.2007.00981.x.
In Switzerland, a national database with 1028 Campylobacter isolates from poultry, pigs, cats, dogs, cattle, humans, zoo animals and water has been created. The database contains the genetic fingerprint and background information of each Campylobacter isolate. Dominant species could be identified in the different sources with a majority of Campylobacter jejuni in poultry (73%), humans (79%), cattle (95%), zoo animals (40%) and water (100%), of Campylobacter coli in pigs (72%), and of Campylobacter upsaliensis/helveticus in cats and dogs (55%). The comparison of three genotyping methods, amplified fragment length polymorphism (AFLP), pulsed field gel electrophoresis and restriction fragment length polymorphism, revealed that AFLP allows discrimination between the different Campylobacter species and is the most appropriate method to distinguish specific strains within the same species. Genotyping analysis demonstrated that the Campylobacter population is heterogeneous among the different sources and that no dominant clone is spread in the country. Genotyping and the resulting database are useful tools to trace back future Campylobacter infections.
在瑞士，已创建了一个包含来自家禽、猪、猫、狗、牛、人类、动物园动物和水的1028株弯曲杆菌分离株的国家数据库。该数据库包含每株弯曲杆菌分离株的基因指纹和背景信息。在不同来源中可以鉴定出优势菌种，在家禽（73%）、人类（79%）、牛（95%）、动物园动物（40%）和水（100%）中多数为空肠弯曲菌，在猪中多数为大肠弯曲菌（72%），在猫和狗中多数为乌普萨拉弯曲菌/瑞士弯曲菌（55%）。对三种基因分型方法，即扩增片段长度多态性（AFLP）、脉冲场凝胶电泳和限制性片段长度多态性的比较显示，AFLP能够区分不同的弯曲杆菌菌种，并且是区分同一菌种内特定菌株的最合适方法。基因分型分析表明，弯曲杆菌群体在不同来源之间是异质的，并且该国没有优势克隆传播。基因分型和由此产生的数据库是追溯未来弯曲杆菌感染的有用工具。
