Niederberger Thomas D, Götz Dorothee K, McDonald Ian R, Ronimus Ron S, Morgan Hugh W
Thermophile Research Unit, University of Waikato, Private Bag 3105, Hamilton, New Zealand.
Department of Biological Sciences, University of Waikato, Private Bag 3105, Hamilton, New Zealand.
Int J Syst Evol Microbiol. 2006 May;56(Pt 5):965-971. doi: 10.1099/ijs.0.63899-0.
Consortia containing a novel coccus-shaped, anaerobic heterotroph together with Pyrobaculum rods were cultivated from geothermal environments in New Zealand. Pure cultures of the cocci were only obtained from one such consortium, despite extensive attempts. Cells of this strain (AQ1.S1T) were regular to irregular cocci in morphology and occasionally formed large aggregates, especially when utilizing polysaccharides such as konjac glucomannan as a carbon source. Strain AQ1.S1T is a hyperthermophile, with an optimal temperature for growth between 92 and 95 degrees C (range 85-98 degrees C), and a moderate acidophile, with optimal growth occurring at pH 6.4 (range 5.4-7.0). Growth was inhibited by the addition of sulphur and NaCl (optimal growth occurred without addition of NaCl) and an electron acceptor was not required. Strain AQ1.S1T utilized starch, trypticase peptone, lactose, glucose, konjac glucomannan, mannose, galactose, maltose, glycogen and beta-cyclodextrin as carbon sources. The G+C content was 52.9 mol%. Based on 16S rRNA gene sequence analysis and physiological features it is proposed that isolate AQ1.S1T (=DSM 17230T=JCM 13409T) represents the type strain of a novel species of a new genus within the Crenarchaeota, Ignisphaera aggregans gen. nov., sp. nov.
从新西兰的地热环境中培养出了含有一种新型球状厌氧异养菌和嗜火栖热菌杆菌的聚生体。尽管进行了大量尝试，但仅从一个这样的聚生体中获得了球菌的纯培养物。该菌株（AQ1.S1T）的细胞形态为规则到不规则的球菌，偶尔会形成大的聚集体，尤其是在利用多糖（如魔芋葡甘聚糖）作为碳源时。菌株AQ1.S1T是嗜热菌，生长的最适温度在92至95摄氏度之间（范围为85 - 98摄氏度），也是中度嗜酸菌，最适生长pH为6.4（范围为5.4 - 7.0）。添加硫和氯化钠会抑制生长（不添加氯化钠时生长最佳），且不需要电子受体。菌株AQ1.S1T利用淀粉、胰蛋白酶蛋白胨、乳糖、葡萄糖、魔芋葡甘聚糖、甘露糖、半乳糖、麦芽糖、糖原和β - 环糊精作为碳源。G + C含量为52.9摩尔%。基于16S rRNA基因序列分析和生理特征，提议将菌株AQ1.S1T（=DSM 17230T =JCM 13409T）作为泉古菌门中一个新属的新物种的模式菌株，命名为聚集火球菌属新属，聚集火球菌新种。
