Gomes-Ruiz Alessandra Cristina, Nascimento Renata Teodoro, de Paula Sérgio Oliveira, Lopes da Fonseca Benedito Antônio
Department of Internal Medicine, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
J Med Virol. 2006 Jun;78(6):760-3. doi: 10.1002/jmv.20620.
Dengue is the most important arthropod-borne viral disease in the world. A rapid diagnostic test for dengue is warranted, and real-time polymerase chain reaction may improve diagnosis. TaqMan and Sybr Green systems were evaluated for the diagnosis of dengue virus type 3 (DENV-3) infections. Out of 77 patients with clinical suspicion of dengue infection, specific IgM antibodies were detected in 40 patients. DENV-3 was detected and quantitated in 17 IgM-positive samples by both systems. These assays were shown to be rapid, and specific for detection of DENV-3, and that for diagnostic purposes, there is no difference between these two assays.
登革热是世界上最重要的节肢动物传播的病毒性疾病。一种用于登革热的快速诊断测试是必要的，实时聚合酶链反应可能会改善诊断。对TaqMan和Sybr Green系统进行了评估，以诊断3型登革热病毒（DENV-3）感染。在77例临床怀疑感染登革热的患者中，40例检测到特异性IgM抗体。两种系统在17份IgM阳性样本中均检测到并定量了DENV-3。这些检测方法显示出快速，且对检测DENV-3具有特异性，并且就诊断目的而言，这两种检测方法之间没有差异。
