Yoshida K, Hirai K, Ara T, Ito M, Wang P-L, Igarashi Y
Department of Removable Prosthodontics, Matsumoto Dental University, Nagano, Japan.
J Oral Rehabil. 2006 May;33(5):363-7. doi: 10.1111/j.1365-2842.2005.01568.x.
The feasibility of using collagen as the base of miconazole was investigated. The addition of 33% collagen to a miconazole solution did not affect the minimal inhibitory concentration (MIC80) of the miconazole solution for Candida albicans. When 1 microg mL(-1) of miconazole in 33% collagen solution was plated on resin discs and dried to yield a thin membrane, the growth of C. albicans on the resin discs was nearly completely inhibited. In addition, we compared the antifungal effect of this collagen solution that contained 1 microg mL(-1) miconazole, with the antifungal effect of miconazole gel that had been diluted with glycerol (the main component of miconazole gel) to yield a final concentration of 1 microg mL(-1) of miconazole; as a result, we found that the collagen solution containing 1 microg mL(-1) miconazole had a stronger antifungal effect. In conclusion, our results demonstrated that it may be feasible to use collagen as the base of miconazole instead of glycerol, and suggest that a collagen-based miconazole solution would have a stronger antifungal effect than commercially available miconazole gel. Collagen-based miconazole solution may be useful for the treatment of Candida-associated denture stomatitis.
研究了使用胶原蛋白作为咪康唑基质的可行性。向咪康唑溶液中添加33%的胶原蛋白不会影响该咪康唑溶液对白色念珠菌的最低抑菌浓度（MIC80）。当将1μg/mL的咪康唑加入33%的胶原蛋白溶液中，铺在树脂圆盘上并干燥以形成薄膜时，树脂圆盘上白色念珠菌的生长几乎被完全抑制。此外，我们将含有1μg/mL咪康唑的这种胶原蛋白溶液的抗真菌效果，与用甘油（咪康唑凝胶的主要成分）稀释至最终浓度为1μg/mL咪康唑的咪康唑凝胶的抗真菌效果进行了比较；结果发现，含有1μg/mL咪康唑的胶原蛋白溶液具有更强的抗真菌效果。总之，我们的结果表明，用胶原蛋白替代甘油作为咪康唑的基质可能是可行的，并表明基于胶原蛋白的咪康唑溶液比市售咪康唑凝胶具有更强的抗真菌效果。基于胶原蛋白的咪康唑溶液可能对治疗念珠菌相关性义齿性口炎有用。
