Paniagua Gloria Luz, Monroy Eric, Negrete Erasmo, Vaca Sergio
Facultad de Estudios Superiores Iztacala, UNAM.
Rev Latinoam Microbiol. 2002 Apr-Jun;44(2):65-8.
Eighty Candida albicans strains, isolated from throat of patients at the Universitary Clinic of the Faculty of Superior Studies Iztacala of the National Autonomous University of Mexico, were analyzed. They were identified by microscopic and colony morphologies, germ tube test, and by auxanogram and zimogram. Minimal inhibitory concentrations (MIC) of 5-fluorocytosine, miconazole and amphotericin B were determined by microtiter broth dilution. MIC frequency distribution of 5-fluorocytosine showed a single peak (0.25-8.0 microg/ml), with 65% susceptible strains (MIC < or = 1.0 < or =g/ml) and 35% intermediate susceptible strains (MIC = 1.1-8 microg/ml). MIC frequency distribution of miconazole was threemodal with 6.25% susceptible (MIC = 1.562 microg/ml), 48.75% intermediate susceptible (MIC = 3.125-12.5 microg/ml), and 45% resistant (MIC = 25-50 microg/ml) strains. All strains were susceptible to amphotericin B (MIC= 0.0156-0.125 microg/m). These results shows that amphotericin B was the more active antimycotic, followed by 5-fluorocytosine, against the strains analyzed, and that miconazole was the less effective one.
对从墨西哥国立自治大学伊萨卡拉高等研究学院大学诊所患者咽喉部分离出的80株白色念珠菌菌株进行了分析。通过显微镜和菌落形态、芽管试验、营养谱和酶谱对它们进行了鉴定。采用微量肉汤稀释法测定了5-氟胞嘧啶、咪康唑和两性霉素B的最低抑菌浓度（MIC）。5-氟胞嘧啶的MIC频率分布显示为单峰（0.25 - 8.0微克/毫升），其中65%的菌株敏感（MIC≤1.0微克/毫升），35%的菌株中度敏感（MIC = 1.1 - 8微克/毫升）。咪康唑的MIC频率分布呈三峰型，6.25%的菌株敏感（MIC = 1.562微克/毫升），48.75%的菌株中度敏感（MIC = 3.125 - 12.5微克/毫升），45%的菌株耐药（MIC = 25 - 50微克/毫升）。所有菌株对两性霉素B敏感（MIC = 0.0156 - 0.125微克/毫升）。这些结果表明，在所分析的菌株中，两性霉素B是活性最强的抗真菌药物，其次是5-氟胞嘧啶，而咪康唑效果最差。
