Zucker B-A, Scharf P, Kersten C
Institute of Animal and Environmental Hygiene, Freie Universität Berlin, Fachbereich Veterinärmedizin, Luisenstrasse 56, 10117 Berlin, Germany.
J Vet Med B Infect Dis Vet Public Health. 2006 May;53(4):176-80. doi: 10.1111/j.1439-0450.2006.00934.x.
Inhalation of bioaerosols from animal houses can induce acute inflammatory reactions in the respiratory tract. Determination of the concentration of airborne endotoxins is widely used to characterize this risk. In this study, the activity of bioaerosol samples from a duck-fattening unit to induce interleukin-1beta (IL-1beta) in human blood and to react with Limulus Amebocyte Lysate (LAL) was investigated. The activity detected in the whole blood assay correlated well with the endotoxic activity found in the LAL assay (Spearmen's rho = 0.902). However in all samples, the inflammation-inducing potential was overestimated by the LAL assay. It is assumed that this overestimation could be, in part, a result of an overestimation of the inflammatory potential of endotoxins originating from Pseudomonadaceae by the LAL assay. Pseudomonadaceae were regularly isolated from the air of the duck-fattening unit. The results presented here indicate that the whole blood assay can be used besides the LAL assay as an additional method to characterize the inflammation-inducing potential of bioaerosols.
吸入动物饲养舍的生物气溶胶可诱发呼吸道急性炎症反应。测定空气中内毒素浓度被广泛用于评估这种风险。在本研究中，对来自一个肉鸭育肥场的生物气溶胶样本在人血液中诱导白细胞介素-1β（IL-1β）以及与鲎试剂（LAL）反应的活性进行了研究。全血试验中检测到的活性与LAL试验中发现的内毒素活性密切相关（Spearmen相关系数ρ = 0.902）。然而，在所有样本中，LAL试验高估了诱导炎症的潜力。据推测，这种高估可能部分是由于LAL试验高估了源自假单胞菌科的内毒素的炎症潜力。在肉鸭育肥场的空气中经常分离出假单胞菌科。此处给出的结果表明，除LAL试验外，全血试验可作为一种补充方法来表征生物气溶胶诱导炎症的潜力。
