Harrison Caroline A, Heaton Martin J, Layton Christopher M, Mac Neil Sheila
Skin Research Unit, Section of Human Metabolism, Division of Clinical Sciences, University of Sheffield, Sheffield, United Kingdom.
Wound Repair Regen. 2006 Mar-Apr;14(2):203-9. doi: 10.1111/j.1743-6109.2006.00111.x.
To produce a stable epidermis, keratinocytes need to be firmly attached to the basement membrane. However, following wounding, keratinocytes are required to develop a migratory phenotype in order to reepithelialize the wound. To investigate some of the issues underlying reepithelialization, we have developed a three-dimensional in vitro model of tissue-engineered skin, comprising sterilized human dermis seeded with human keratinocytes and dermal fibroblasts. Using this model, we have shown that the inclusion of fibroblasts within the model increases the stability of keratinocyte attachment. We have also demonstrated that keratinocyte migration occurs most effectively in the absence of a basement membrane and following the inclusion of fibroblasts in the model. In addition, subjecting the keratinocyte layer to mechanical trauma induces a migratory phenotype. We conclude that this three-dimensional in vitro wound model can be used to increase our understanding of the factors that enhance keratinocyte migration and hence wound healing in vivo.
为了形成稳定的表皮，角质形成细胞需要牢固地附着于基底膜。然而，在受伤后，角质形成细胞需要形成迁移表型以便伤口重新上皮化。为了研究再上皮化背后的一些问题，我们构建了一种组织工程皮肤的三维体外模型，该模型由接种了人角质形成细胞和真皮成纤维细胞的灭菌人真皮组成。利用该模型，我们发现模型中包含成纤维细胞可增加角质形成细胞附着的稳定性。我们还证明，在没有基底膜且模型中包含成纤维细胞的情况下，角质形成细胞迁移最为有效。此外，对角质形成细胞层施加机械创伤可诱导迁移表型。我们得出结论，这种三维体外伤口模型可用于增进我们对促进角质形成细胞迁移从而促进体内伤口愈合的因素的理解。
