Nosek Jozef, Tomaska Lubomir, Bolotin-Fukuhara Monique, Miyakawa Isamu
Department of Biochemistry, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia.
FEMS Yeast Res. 2006 May;6(3):356-70. doi: 10.1111/j.1567-1364.2005.00016.x.
Recent progress in the analysis of protein components of the mitochondrial nucleoid and replisome of baker's yeast, Saccharomyces cerevisiae, opens a unique opportunity for understanding the molecular principles of mitochondrial inheritance. In this work we identified homologs of proteins involved in the mitochondrial DNA packaging and replication in the complete genome sequence of the petite-negative yeast Kluyveromyces lactis. Comparative analysis of their counterparts from phylogenetically diverse yeast species revealed conserved as well as diverged features of the organellar chromosome structure and its replication strategy. Moreover, it provides a basis for subsequent functional studies of the structure and dynamics of the mitochondrial nucleoids.
对酿酒酵母线粒体类核和复制体蛋白质成分分析的最新进展，为理解线粒体遗传的分子原理提供了独特契机。在这项研究中，我们在小菌落阴性酵母乳酸克鲁维酵母的全基因组序列中鉴定出参与线粒体DNA包装和复制的蛋白质同源物。对来自系统发育上不同酵母物种的对应物进行比较分析，揭示了细胞器染色体结构及其复制策略的保守和差异特征。此外，它为后续对线粒体类核的结构和动态进行功能研究提供了基础。
