Ouzounis C, Bork P, Casari G, Sander C
European Molecular Biology Laboratory-Heidelberg, Germany.
Protein Sci. 1995 Nov;4(11):2424-8. doi: 10.1002/pro.5560041121.
The analysis of the 269 open reading frames of yeast chromosome VIII by computational methods has yielded 24 new significant sequence similarities to proteins of known function. The resulting predicted functions include three particularly interesting cases of translation-associated proteins: peptidyl-tRNA hydrolase, a ribosome recycling factor homologue, and a protein similar to cytochrome b translational activator CBS2. The methodological limits of the meaningful transfer of functional information between distant homologues are discussed.
通过计算方法对酵母八号染色体的269个开放阅读框进行分析，发现了24个与已知功能蛋白质具有新的显著序列相似性的结果。由此预测的功能包括三个特别有趣的与翻译相关的蛋白质案例：肽基-tRNA水解酶、核糖体循环因子同源物以及一种类似于细胞色素b翻译激活因子CBS2的蛋白质。本文还讨论了在远缘同源物之间进行功能信息有意义传递的方法学局限性。
