Nishitani Yoshihiro, Yoshiyama Masahiro, Wadgaonkar Bakul, Breschi Lorenzo, Mannello Ferdinando, Mazzoni Annalisa, Carvalho Ricardo M, Tjäderhane Leo, Tay Franklin R, Pashley David H
Department of Operative Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Japan.
Eur J Oral Sci. 2006 Apr;114(2):160-6. doi: 10.1111/j.1600-0722.2006.00342.x.
Mild acids are known to activate dentin matrix metalloproteinase (MMPs). All self-etching dental adhesives are acidic (pH 1.5-2.7) and may activate dentin MMPs. The purpose of this study was to compare the ability of several all-in-one adhesives to activate gelatinolytic and collagenolytic activities in powdered mineralized dentin. Powdered dentin made from human teeth was mixed with all-in-one adhesives (Clearfil Tri-S Bond, G-Bond, Adper Prompt L-Pop) or a self-etching primer (Clearfil SE Bond primer) for varying times and then the reaction was stopped by extracting the adhesives using acetone. Fresh untreated mineralized dentin powder had a gelatinolytic activity of 3.31 +/- 0.39 relative fluorescent units (RFU) per mg dry weight (24 h) that increased, over storage time, to 87.5 RFU mg(-1) (24 h) after 6-8 wk. When fresh powder was treated with acidic Tri-S Bond, the gelatinolytic activity increased from 3.24 +/- 0.70 RFU mg(-1) to > 112.5 RFU mg(-1) (24 h) after 20 min and then remained unchanged. Monomers with lower pH values produced less activity. There was a significant, direct correlation between gelatinolytic activity and pH, with Tri-S giving the highest activity. Coating dentin powder with Tri-S resin prevented fluorescent substrates from gaining access to the enzyme, even though it activated the enzyme. In conclusion, self-etch adhesives may activate latent MMP and increase the activity to near-maximum levels and contribute to the degradation of resin-dentin bonds over time.
已知弱酸可激活牙本质基质金属蛋白酶（MMPs）。所有自酸蚀牙科黏结剂均呈酸性（pH值为1.5 - 2.7），可能会激活牙本质MMPs。本研究的目的是比较几种一体化黏结剂激活矿化牙本质粉中明胶溶解活性和胶原溶解活性的能力。将人牙制成的牙本质粉与一体化黏结剂（Clearfil Tri-S Bond、G-Bond、Adper Prompt L-Pop）或自酸蚀底漆（Clearfil SE Bond底漆）混合不同时间，然后用丙酮提取黏结剂以终止反应。新鲜未处理的矿化牙本质粉每毫克干重（24小时）的明胶溶解活性为3.31±0.39相对荧光单位（RFU），在储存6 - 8周后，该活性随储存时间增加至87.5 RFU mg⁻¹（24小时）。当用酸性的Tri-S Bond处理新鲜牙本质粉时，20分钟后明胶溶解活性从3.24±0.70 RFU mg⁻¹增加至＞112.5 RFU mg⁻¹（24小时），然后保持不变。pH值较低的单体产生的活性较低。明胶溶解活性与pH值之间存在显著的直接相关性，其中Tri-S的活性最高。用Tri-S树脂包被牙本质粉可防止荧光底物接触到酶，尽管它激活了该酶。总之，自酸蚀黏结剂可能激活潜在的MMP，并将活性提高到接近最大值的水平，随着时间的推移会导致树脂 - 牙本质黏结的降解。
