Borges Ana Flávia Sanches, Puppin-Rontani Regina Maria, Bittar Renata Andrade, Kantowitz Kamila Rosamilia, Pascon Fernanda Miori, Martin Airton Abrahão
Latin American Institute of Research and Teaching in Dentistry, Brazil.
Am J Dent. 2009 Feb;22(1):30-6.
To evaluate the quality of primary and permanent dentin by Fourier transformed Raman spectroscopy (FT-Raman), and scanning electron microscopy/energy-dispersive spectroscopy (SEM/EDS).
The middle dentin of crowns was reached by carbide bur abrading providing a uniform smear layer. Self-etching primers were applied in order to simulate the etching of self-etching adhesive systems. The groups were (n = 6): G1 (primary dentin smear layer); G2 (primary dentin etched by primer of Clearfil Protect Bond); G3 (primary dentin etched by Adper Prompt); G4 (permanent dentin smear layer); G5 (permanent dentin etched by primer of Clearfil Protect Bond); G6 (permanent dentin etched by Adper Prompt). SEM/EDS were made in order to obtain additional elemental data to complement FT-Raman. FT-Raman data were submitted to cluster analysis.
Overall, FT-Raman showed differences between primary and permanent dentin concerning organic content, but not for inorganic content. FT-Raman showed differences in the organic content between primary and permanent dentin after self-etching primer use. HEMA usage caused molecular changes in the organic content, while phosphoric acidic ester caused molecular changes in the inorganic content of primary and permanent dentin. The SEM/EDS identified C, O, P, and Ca, which could not replace ions to change mineral molecular arrangement. Both organic and mineral content arrangements were similar after self-etching primers action. The organic content of dentin was modified by both Clearfil PB primer and Adper Prompt for primary dentin but only by Clearfil PB for permanent dentin. The inorganic content of primary middle dentin was similar to the inorganic content of permanent middle dentin, even when Adper Prompt is used.
通过傅里叶变换拉曼光谱（FT - Raman）以及扫描电子显微镜/能谱分析（SEM/EDS）评估乳牙和恒牙牙本质的质量。
用硬质合金车针磨除牙冠中部牙本质以形成均匀的玷污层。应用自酸蚀底漆以模拟自酸蚀粘结系统的酸蚀过程。分组如下（n = 6）：G1（乳牙玷污层）；G2（用Clearfil Protect Bond底漆酸蚀的乳牙牙本质）；G3（用Adper Prompt酸蚀的乳牙牙本质）；G4（恒牙玷污层）；G5（用Clearfil Protect Bond底漆酸蚀的恒牙牙本质）；G6（用Adper Prompt酸蚀的恒牙牙本质）。进行SEM/EDS分析以获取补充FT - Raman的额外元素数据。对FT - Raman数据进行聚类分析。
总体而言，FT - Raman显示乳牙和恒牙牙本质在有机成分方面存在差异，但在无机成分方面无差异。使用自酸蚀底漆后，FT - Raman显示乳牙和恒牙牙本质的有机成分存在差异。使用甲基丙烯酸羟乙酯（HEMA）导致有机成分发生分子变化，而磷酸酸性酯导致乳牙和恒牙牙本质的无机成分发生分子变化。SEM/EDS鉴定出碳（C）、氧（O）、磷（P）和钙（Ca），它们无法置换离子来改变矿物分子排列。自酸蚀底漆作用后，有机和矿物成分排列相似。Clearfil PB底漆和Adper Prompt均能改变乳牙牙本质的有机成分，但仅Clearfil PB能改变恒牙牙本质的有机成分。即使使用Adper Prompt，乳牙中部牙本质的无机成分与恒牙中部牙本质的无机成分相似。
