Zhang Long-Hua, Xu Lin, Xu Tian-Le
School of Life Sciences, University of Science and Technology of China, Hefei 230027, China.
Biochem Biophys Res Commun. 2006 Jun 9;344(3):721-6. doi: 10.1016/j.bbrc.2006.03.198. Epub 2006 Apr 17.
Functional glycine receptors (GlyRs) are enriched in the hippocampus, but their roles in synaptic transmission are unclear. In this study, we examined the effect of GlyR activation on paired-pulse stimulation of the whole-cell postsynaptic currents (PSCs) in the Schaffer-CA1 synapses in rat hippocampal slices. Bath application of glycine reduced the amplitude of PSCs, accompanied by an increase in holding current and resting conductance. Moreover, glycine application increased the paired-pulse ratio (PPR) of PSCs significantly, an effect largely abolished by the GlyR specific antagonist strychnine. Interestingly, glycine application had no significant effect on either the amplitude or the PPR of excitatory postsynaptic currents (EPSCs). Our findings suggest that GlyR activation regulates hippocampal short-term plasticity by altering GABAergic neurotransmission.
功能性甘氨酸受体（GlyRs）在海马体中含量丰富，但其在突触传递中的作用尚不清楚。在本研究中，我们检测了甘氨酸受体激活对大鼠海马体切片中Schaffer-CA1突触全细胞突触后电流（PSCs）配对脉冲刺激的影响。浴灌甘氨酸降低了PSCs的幅度，同时伴有钳制电流和静息电导的增加。此外，应用甘氨酸显著增加了PSCs的配对脉冲比率（PPR），甘氨酸受体特异性拮抗剂士的宁可在很大程度上消除这一效应。有趣的是，应用甘氨酸对兴奋性突触后电流（EPSCs）的幅度或PPR均无显著影响。我们的研究结果表明，甘氨酸受体激活通过改变GABA能神经传递来调节海马体的短期可塑性。
