给予d-苯丙胺后大鼠海马区星形胶质细胞增生的证据。
Evidence of astrogliosis in rat hippocampus after d-amphetamine exposure.
作者信息
Frey Benício N, Andreazza Ana C, Ceresér Keila M, Martins Márcio R, Petronilho Fabrícia C, de Souza Daniela F, Tramontina Francine, Gonçalves Carlos A, Quevedo João, Kapczinski Flávio
机构信息
Department of Biochemistry, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
出版信息
Prog Neuropsychopharmacol Biol Psychiatry. 2006 Sep 30;30(7):1231-4. doi: 10.1016/j.pnpbp.2006.03.016. Epub 2006 Apr 21.
INTRODUCTION
Psychostimulants such as amphetamine (AMPH) induce manic-like symptoms in humans and studies have suggested that bipolar disorder (BD) may be associated to dopamine dysfunction. Glial fibrillary acidic protein (GFAP) up-regulation is considered a marker of astrogliosis, and it has been associated to behavioral sensitization.
PURPOSE
We aimed to investigate the behavioral effects of acute and chronic AMPH on rat locomotion and assess GFAP levels in rat cortex and hippocampus.
METHODS
Rats were administered either acute (single dose) or chronic (seven days) d-amphetamine IP injection. Locomotion was assessed with an open-field test and GFAP immunoquantity was measured using ELISA.
RESULTS
Chronic, but not acute, administration of AMPH increased GFAP levels in rat hippocampus. No differences were observed in rat cortex.
CONCLUSIONS
Repeated exposure to AMPH leads to an astroglial response in the hippocampus of rats.
引言
苯丙胺（AMPH）等精神兴奋剂会在人类身上诱发类似躁狂的症状，并且研究表明双相情感障碍（BD）可能与多巴胺功能障碍有关。胶质纤维酸性蛋白（GFAP）上调被认为是星形胶质细胞增生的一个标志，并且它与行为敏化有关。
目的
我们旨在研究急性和慢性AMPH对大鼠运动的行为影响，并评估大鼠皮质和海马体中的GFAP水平。
方法
给大鼠腹腔注射急性（单剂量）或慢性（七天）的右旋苯丙胺。通过旷场试验评估运动情况，并使用酶联免疫吸附测定法测量GFAP免疫量。
结果
慢性而非急性给予AMPH会增加大鼠海马体中的GFAP水平。在大鼠皮质中未观察到差异。
结论
反复接触AMPH会导致大鼠海马体出现星形胶质细胞反应。
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