Lim Roderick Y H, Fahrenkrog Birthe
M.E. Müller Institute for Structural Biology, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland.
Curr Opin Cell Biol. 2006 Jun;18(3):342-7. doi: 10.1016/j.ceb.2006.03.006. Epub 2006 May 2.
Transport between the nucleus and cytoplasm is mediated by nuclear pore complexes (NPCs), perforations in the double-membrane of the nuclear envelope. NPCs are huge protein assemblies made up of distinct subcomplexes. The complex modular nature of the NPC and limitations in the current experimental approaches render the analysis of NPCs and nucleocytoplasmic transport at the molecular level difficult. Recent efforts in the NPC/nucleocytoplasmic transport field have focused on elucidating the core components that make up NPC structure (or the lack thereof) and function. These include results obtained by more conventional methods, such as electron microscopy or biochemical strategies, as well as more advanced applications, such as X-ray crystallography and atomic force microscopy.
细胞核与细胞质之间的运输由核孔复合体（NPCs）介导，核孔复合体是核膜双层膜上的穿孔。核孔复合体是由不同亚复合体组成的巨大蛋白质集合体。核孔复合体复杂的模块化性质以及当前实验方法的局限性使得在分子水平上分析核孔复合体和核质运输变得困难。核孔复合体/核质运输领域最近的工作重点是阐明构成核孔复合体结构（或缺乏结构）和功能的核心成分。这些成果包括通过更传统的方法获得的结果，如电子显微镜或生化策略，以及更先进的应用，如X射线晶体学和原子力显微镜。
